DNA
Part:BBa_K1510087:Design
Designed by: Kao Jung Chang Group: iGEM14_NYMU-Taipei (2014-10-08)
Fragrance generator part1: ketoisocaproate decarboxylase (THI3)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 3
Illegal EcoRI site found at 904
Illegal EcoRI site found at 1603
Illegal EcoRI site found at 1789 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 3
Illegal EcoRI site found at 904
Illegal EcoRI site found at 1603
Illegal EcoRI site found at 1789 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 3
Illegal EcoRI site found at 904
Illegal EcoRI site found at 1603
Illegal EcoRI site found at 1789 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 3
Illegal EcoRI site found at 904
Illegal EcoRI site found at 1603
Illegal EcoRI site found at 1789 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 3
Illegal EcoRI site found at 904
Illegal EcoRI site found at 1603
Illegal EcoRI site found at 1789 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
To fit igem part standard, the circuit is constructed in backbone pSB1C3, chloramphenicol with EcoR1 and Xba1 in front of the part, and Spe1, Pst1 behind the part.
Source
yeast genomic DNA